10/08/2008

Human Immunodeficiency Virus (HIV) - In vitro antiviral activity

• In vitro Antiviral Activity ( anti- H.I.V-1)

Experimental design

 

P-MAPA dissolved in dimethylsulfoxide was added to T4 lymphocyte culture (CEM-IW strain)(range of 1.8 x 10?6 to 5.7 x 10-10 M) and then added to HIV-1.

 

Uninfected and infected cells without P-MAPA treatment were used as basic control.

 

Essentially, the test involve the T4 lymphocyte death, by HIV-1.

 

Results :

 

The experiments showed that P-MAPA is inactive against HIV-1 virus in vitro.

 

• P-MAPA Effect on HIV-1 Virus in Infected Cells ( viral replication)

 

HIV- T4 lynphocyte culture

 

The experiment was carried out in 5 ml of 8 x 105 T4 lymphocyte culture (CEM cells) in CIM and infected with 103 TCID50 of HIV-Ib .

 

P-MAPA did not interfere with the CEM viability and p55 ELISA test showed to be the same dosage than the control samples

 

Conclusion : P-MAPA has no effect on virus production ( viral replication) when added to infected CEM cells.